A Study on the Effects of Lonicera japonica Components on Bone Formation
Kim Sin-Guen, Park Byeong-Gi, 홍남희, 공윤수, 김용수, Kwon Tai-Gyu, 박준우, 최동주,
소속 상세정보
( Kim Sin-Guen ) - Hallym University Graduate School Department of Medical Science Maxillofacial Implantology
( Park Byeong-Gi ) - Hallym University Kangdong Sacred Heart Hospital Department of Oral and Maxillofacial Surgery
홍남희 ( Hong Nam-Hee ) - Hallym University Graduate School Department of Medical Science Maxillofacial Implantology
공윤수 ( Gong Yoon-Soo ) - Hallym University Graduate School Department of Medical Science Maxillofacial Implantology
김용수 ( Kim Yong-Su ) - Hallym University Graduate School Department of Medical Science Maxillofacial Implantology
( Kwon Tai-Gyu ) - Hallym University Graduate School Department of Medical Science Maxillofacial Implantology
박준우 ( Park Jun-Woo ) - Hallym University Kangdong Sacred Heart Hospital Department of Oral and Maxillofacial Surgery
최동주 ( Choi Dong-Ju ) - Hallym University Kangdong Sacred Heart Hospital Department of Oral and Maxillofacial Surgery
KMID : 1157520150110030177
Abstract
Objective: In case of placing implant on infectious circumstances may be occurred which is the main causes of implant failure. Because of this reason, surgeons have stresses to control infections during surgery. We used the Lonicera japonica abstract for this purpose and want to find the real effect of L. Japonica on infectious situation.
Methods: Evaluating the toxicity and bone forming ability of luteolin, the MTT assay was carried out with a concentration of luteolin used in previous studies evaluating cytotoxicity in osteoblasts. And also alkaline phosphatase (ALP) activity with MG63 cells were used to evaluate the increase in ALP activity. Total number of 28 implants Implant was placed in 14 rabbits tibia and to check bone formation activity, histological test and removable torque test was also done. Control group: implant placement without treatment of the bone defect area. Experimental group 1: implant placement with bone graft material. Experimental group 2: implant placement with bone graft material after infection with Staphylococcus aureus. Experimental group 3: implant placement with bone graft material after infection with S. aureus and luteolin treatment.
Results: We found that there is a significant difference between the mean value of implant removal force (removal torque) for each group (p=0.007). The amount of bone formation in experimental groups 2 and 3 were significantly different which suggested that luteolin enhanced bone formation in the presence of bone graft infection.
Conclusion: In this study we investigated about the luteolin, which is one of the anti-microbial and anti-inflammatory components of L. japonica, that it’s activity on promotion of bone formation when it was applied to infectious circumstances. And we also investigate the possibility of application of luteolin to human cells.
키워드
Lonicera japonica; luteolin; bone formation; anti-microbial; anti-inflammatory
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